Figure 6
From: Buprenorphine alters microglia and astrocytes acutely following diffuse traumatic brain injury
Astrocytes are larger with Bup-treatment in the thalamus, but not in the cortex. Representative photomicrographs of the astrocytic glial fibrillary acidic protein (GFAP) in (A,B) cortex and (C,D) thalamus from rats 1d post-cFPI and treated with (A,C) saline or (B,D) buprenorphine (Bup). Bar graphs depicting astrocyte (E) cell number, (F) % of GFAP + coverage/image, (G) average astrocyte cell size, and (H) astrocyte cell circularity. There were more, larger GFAP + astrocytes covering a greater area in the thalamus compared to the cortex of saline treated rats 1d following cFPI. Thalamic astrocytes were larger with Bup treatment compared to those in saline treated rats, however cortical astrocyte size was not significantly altered by Bup treatment. Figure was compiled using Adobe Photoshop CS., version 22.0 (2020), San Diego, CA. Treatment differences: *p < 0.05 compared to saline; regional differences: #p < 0.05 compared to cortex. n = 6 rats/group. Mean ± SEM. Scale = 50 μm.
