Figure 7
From: Buprenorphine alters microglia and astrocytes acutely following diffuse traumatic brain injury
Acute myelin debris and MBP expression was unchanged with Bup treatment but was different between cortex and thalamus. Representative photomicrographs of myelin integrity in (A,B) cortex treated with saline or buprenorphine (Bup) and (C,D) thalamus treated with saline or Bup, analyzed using ImageJ. Representative bands for (E) Western blot analysis of actin protein (band at ~ 40 kDa) and myelin basic protein (MBP; bands at ~ 15–20 kDa). (F) Bar graph depicting average overall MBP expression (gray bars), as well as expression of 21.5 kDa (red bars), 18.5 kDa (orange bars), 17.2 kDa (blue bars), and 14.0 kDa (green bars) MBP isoforms in the cortex and thalamus of saline and Bup treated animals. Overall MBP expression and expression of individual MBP isoforms were not significant in either saline or Bup treatment groups. There were, however, significant regional differences with higher MBP expression in the cortex than in the thalamus. (G) Bar graph depicting average number of MBP fibers and MBP debris of cortex and thalamus in saline and Bup treated animals. The number of myelinated fibers was consistent between groups, indicating neither region was affected by buprenorphine treatment. Figure was compiled using Adobe Photoshop CS., version 22.0 (2020), San Diego, CA. Treatment differences: regional differences: #p < 0.05 compared to cortex. n = 6 rats/group. Mean ± SEM. Scale = 50 μm.
