Figure 2 | Scientific Reports

Figure 2

From: C subunit of the ATP synthase is an amyloidogenic calcium dependent channel-forming peptide with possible implications in mitochondrial permeability transition

Figure 2

Aggregation properties of the c subunit. (A) Thioflavin T fluorescence spectra showing calcium dependence of c subunit aggregation (created with OriginPro 2021, OriginLab Corp. Northampton, MA); (B) SDS PAGE of the c subunit showing presence of oligomeric and monomeric forms; (C) 5 × 5 µm AFM image of c subunit fibrils after incubation at 37 °C for 4 h in 2% Genapol and PBS; (D) 5 × 5 µm AFM image of the c subunit oligomers (arrows) after incubation at 37 °C for 4 h and deposited on mica. Below each image is provided the height profile of the indicated white line; (E) Dot blot of the c subunit incubated with anti c subunit and A11-19 antibodies with and without Ca2+ showing the ability of the protein to form cross-β oligomers (full-length blots are presented in Supplementary Figure 1).

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