Figure 2 | Scientific Reports

Figure 2

From: Coiled-coil heterodimers with increased stability for cellular regulation and sensing SARS-CoV-2 spike protein-mediated cell fusion

Figure 2

Reconstitution of split luciferase can be tuned by CC stability. Luciferase activity was determined 48 h after transfection of HEK293T cells with plasmids expressing nLuc:N8 or nluc:N6 (30 ng); and cLuc tethered to N7, P7A, P7, P7SN or to N5, P5A, P5, P5SN (10–90 ng). (A, B) Increasing amounts of plasmids expressing cLuc:CC resulted in increased luciferase activity. (C, D) Only orthogonal peptide variants with complementary electrostatic motifs formed functional luciferase. HEK293T cells were transfected with plasmids expressing nLuc:N8 or nluc:N6 (30 ng); and cLuc tethered to N7, P7A, P7, P7SN or to N5, P5A, P5, P5SN (10 ng).The values represent the means (± s.d.) from four independent cell cultures, individually transfected with the same mixture of plasmids, and are representative of two independent experiments. Amounts of used plasmids are indicated in Table S2.

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