Table 2 Description of the plasmids present in the 8 ETEC reference strains.
Plasmid | Length (bp) | GC (%) | Inca | Plasmid features (no of copies) | Virulence genes | Putative virulence genes | Antibiotic resistance profile (genomic) | Acc. no | |
|---|---|---|---|---|---|---|---|---|---|
L1 E925 CS1 + CS3 + CS21 LTh + STh | pAvM_E925_4 | 116 803 | 48.4 | FII | ccdAB, hok-sok (antisense RNA-regulated system), psiAB, repAB, stbAB, tra genes | cstA-G (CS3), eltAB1 (LTh), estA3/4 (STh) | etpBAC | – | LR883051 |
pAvM_E925_5 | 82 909 | 48.6 | FII + FIB | psiAB, repA (2), repB, repE, parB, sopAB, tra genes | lngX1, R, S, T, X2, A-J, P (CS21) | – | – | LR883052 | |
pAvM_E925_6 | 82 314 | 47.8 | I1 | iib (colicin 1b), repA, stbAB, tra genes, pil locus, vapBC | cooB, A, C, D (CS1) | cexE | – | LR883053 | |
pAvM_E925_7 | 51 418 | 45.4 | FII | ccdAB, psiA, repAB, stbAB, tra genes | – | eatA_1-5 (two disrupted eatA copies) | – | LR883054 | |
L2 E1649 CS2* + CS3 + CS21 LTh + STh | pAvM_E1649_8 | 120 141 | 47.2 | FII | ccdAB (duplicated), hok-sok (antisense RNA-regulated system), psiAB, repAB, stbAB, tra genes | cstA-G (CS3), eltAB1 (LTh), estA3/4 (STh) | eatA, etpBAC | – | LR882976 |
pAvM_E1649_9* | 102 017 | 47.6 | Y | P1 addiction system (phage related), repA, sopAB | – | – | LR882977 | ||
pAvM_E1649_10 | 86 517 | 45.0 | FII + FIB | hok-sok (antisense RNA-regulated system), psiAB, repA(2), repB, sopAB, tra genes | lngX1, R, S, T, X2, A-J, P (CS21) | – | – | LR882974 | |
pAvM_E1649_11* | 8 834 | 42.9 | No hits | ND | – | – | – | LR882975 | |
L3 E36 CFA/I + CS21 LTh + STh | pAvM_E36_12* | 381 858 | 49.7 | FII + FIB | stbAB (4), psiAB (6), tra genes, repA (3), repB (3), sopAB (2), hok-sok (antisense RNA-regulated system), relE | cfaA, B, C, D (CFA/I), eltAB15 (LTh), lngX1, R, S, T, X2, A-J, P (CS21), estA2 (STh) | eatA, etpBAC | – | LR882998 |
pAvM_E36_13 | 99 448 | 51.6 | B/O/K/Z | stbAB, relE, repA, pil genes, psiAB | – | – | tetA, B, C, R mdf(A)-like | LR882999 | |
L3 E2980 CS7 LTh | pAvM_E2980_14 | 112 056 | 48.1 | I1 | parA, pil genes, relE, repA, stbAB | csvA, B, C, D (CS7), eltAB (LTh) | cexE | – | LR882979 |
pAvM_E2980_15 | 72 255 | 52.4 | FII | psiAB, relE, repAB, stbAB, tra genes | – | – | strA, strB, sul2, blaTEM-1B | LR882980 | |
pAvM_E2980_16 | 48 305 | 50.3 | I1-like | stbAB, repA, vapBC (TA-system) | – | eatA, etpBAC | – | LR882981 | |
L4 E1441 CS6 + CS21 LTh (LT17) | pAvM_E1441_17 | 130 302 | 51.3 | FII + FIB | pemI/K (TA-system), psiAB, repA (2), repB, sopAB, srnAC (antisense RNA-regulated system), stbAB, tra genes | lngX1, R, S, T, X2, A-J, P (CS21) | – | aadA1, tetR, tetA, sul1, dfrh1 | LR883013 |
pAvM_E1441_18 | 94 840 | 47.1 | FII | parB, psiAB, repAB, stbAB | cssA, B, C, D (CS6), eltAB17 (LTh) | etaA_1, eatA_2, cexE | – | LR883014 | |
L5 E1779 CS5 + CS6 LTh + STh | pAvM_E1779_19 | 142 377 | 47.6 | FII | ccdAB (TA-system), cea/cia (Colicin E), psiAB, repAB, stbAB (2 copies), tra genes, vapBC (TA-system) | csfA, B, C, E, F, D (CS5), cssA, B, C, D (CS6), estA3/4 (STh) | eatA_1, eatA_2 | – | LR883008 |
pAvM_E1779_20 | 88 759 | 51.8 | FII | hok-sok (antisense RNA-regulated system), psiAB, tra genes, repAB, stbAB | eltAB15 (LTh) | – | – | LR883009 | |
pAvM_E1779_21 | 82 464 | 51.0 | FIIY | repA (2), repB, tra genes, psiAB, parB, sopAB | – | – | – | LR883010 | |
pAvM_E1779_22 | 61 528 | 50.5 | FII | repAB, tra genes | – | – | LR883011 | ||
L6 E562 CFA/I + CS21 STh | pAvM_E562_23 | 109 853 | 50.5 | I1 (+ FII) | parB, psiAB, stbAB, pil genes, tra genes | – | eatA, etpBAC | – | LR883001 |
pAvM_E562_24 | 86 655 | 48.6 | FII + FIB | psiAB, repA (2), repB, stbAB, tra genes | lngX1, R, S, T, X2, A-J, P (CS21) | – | – | LR883002 | |
pAvM_E562_25* | 81 468 | 46.7 | FII | psiAB (truncated psiA) relE/B (toxin-antitoxin system), repAB, stbAB, sopAB | cfaA, B, C, D (CFA/I), estA2 (STh) | – | – | LR883003 | |
pAvM_E562_26 | 88 318 | 52.9 | B/O/K/Z | pil genes, pndAC (antisense RNA-regulated system) psiAB, relE, repA, tra genes | – | – | – | LR883004 | |
pAvM_E562_27* | 83 375 | 40.0 | FII | hok-sok (antisense RNA-regulated system), pemI/pemK (TA system), psiAB, parB, repAB, tra genes | – | – | blaTEM-1b, tetAR, merRTPCADE (Tn21) | LR883005 | |
L7 E1373 CS6 STp | pAvM_E1373_28 | 146 433 | 46.1 | FII + FIB | parB, psiAB, relE, repA (2), repB | cssA, B, C, D (CS6), estA5 (STp), CS8-like gene cluster, fae-related genes | – | – | LR882991 |
pAvM_E1373_29 | 109 318 | 46.4 | FIB | parB, parB-like, repA | – | – | – | LR882992 |
