Table 1 Antibodies and lectins used, with species of elaboration, dilution, manufacturer, and catalogue number.

From: A comprehensive structural, lectin and immunohistochemical characterization of the zebrafish olfactory system

Ab/lectin*

1st Ab/lectin species and dilution

1st Ab/lectin catalogue number

2nd Ab kit (catalogue number)

Anti-Gαo

Rabbit 1:100

Sta Cruz Biotechnology

SC-387

ImmPRESS VR HRP Anti-Rabbit IgG Reagent MP-6401-15

Anti-Gαi2

Rabbit 1:100

Sta Cruz Biotechnology

SC-7276

ImmPRESS VR HRP Anti-Rabbit IgG Reagent MP-6401-15

Anti-GFAP

Rabbit 1:400

Dako Z0334

ImmPRESS VR HRP Anti-Rabbit IgG Reagent MP-6401-15

Anti-Calbindin

Rabbit 1:5000

Swant CB38

ImmPRESS VR HRP Anti-Rabbit IgG Reagent MP-6401-15

Anti-GAP-43

Mouse 1:400–1:4000

Sigma G9264

ImmPRESS VR HRP Anti-Mouse IgG Reagent MP-6402-15

Anti-Calretinin

Rabbit 1:5000

Swant 7697

ImmPRESS VR HRP Anti-Rabbit IgG Reagent MP-6401-15

Anti-LHRH

Rabbit 1:500

Fisher Scientific A235481

ImmPRESS VR HRP Anti-Rabbit IgG Reagent MP-6401-15

UEA-I*

1:10

Vector L-1060

Rabbit 1:50 DAKO P289

LEA*

20 μg/ml

Vector B-1175

Vectastain ABC reagent PK-4000

BSI-B4*

100 μg/ml

Sigma L-2140

Vectastain ABC reagent PK-4000

  1. Gαo: subunit αo of G protein; Gαi2: subunit αi2 of G protein; OMP: olfactory marker protein; MAP-2: microtubule associated protein-2; GAP-43: growth-associated protein 43; GFAP: glial fibrillary acidic protein; CB: calbindin; CR: calretinin; LHRH: luteinizing hormone-releasing hormone; UEA: Ulex europaeus agglutinin; LEA: Lycopersicum esculentum agglutinin; BSI-B4: Bandeiraea simplicifolia isolectin B4; HRP: horseradish peroxidase; IgG: Immunoglobulin G; ABC: avidin–biotin-complex.
  2. The lectins employed are indicated by an asterisk (*).
Back to article page