Figure 2 | Scientific Reports

Figure 2

From: High sensitivity sanger sequencing detection of BRAF mutations in metastatic melanoma FFPE tissue specimens

Figure 2

Comparative analytical results for select FFPE clinical samples. (a) IHC anti-BRAF V600E stained tissue from patient 1. Stained tumor exhibited brown color as pointed by the arrowhead. (b,c) Results for non-macrodissected patient 1 sample 129282, including BDA qPCR curves, Sanger sequencing trace(b) and ddPCR scatter plot(c). (d,e) Results for macrodissected patient 1 sample 129280. (f–h) IHC, BDA Sanger, and ddPCR results for patient 4 sample 129292. (i–k) IHC, BDA Sanger, and ddPCR results for patient 6 sample 129298. Red qPCR curve showed amplification of BDA reaction with blocker for variant enrichment, and blue curve showed amplification of control reaction that did not contain blocker. The position of variant peak is highlighted in Sanger trace. In ddPCR scatter plots, the lower left quadrant showed empty droplets. The lower right quadrant showed droplets containing WT molecules. The upper left quadrant displayed droplets containing variant molecules. The upper right quadrant exhibited droplets containing both WT and variant molecules.

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