Figure 1

Alterations to gut microbiome in relation to exposure and inflammation. (A) Schematic of transplant experiments in which wild-type (WT) C57BL/6 mice received an aortic arch from an Apoe^−/− donor. One group of WT mice received the aortic arch transplant but were not given antibiotics (Abx⁻). The other group received antibiotic treatment 3 days prior to the aortic arch transplant, and throughout the 5 days post-transplant (Abx⁺). Fecal samples were collected from both groups pre- and post-aortic transplant. Analysis, comparing the antibiotic-exposed WT mice or not, according to: (B) Fecal samples post-aortic transplant; Shannon Diversity Index, and (C) weighted UniFrac analysis, to determine Beta-diversity; (D,E) Relative taxonomic abundance by phylum. In (D), each box represents a fecal sample while in (E), each box represents one mouse with bars indicating individual 16S rRNA fecal sample analysis. ABX⁺ mice had significantly reduced Bacteroidetes after treatment. (n =  ~ 7–8 mice/group) ***P < 0.001. Cartoons (1A) obtained from SMART Servier Medical Art under a Creative Commons Attribution 3.0 Unported License.