Table 2 Top 10 proteins of CSPa per quantitative values (% of normalized total spectral strength of each band sample).
MW, kDa | Ca1 | Ca2 | Ca3 | Ca4 | Ca5 | Ca6 | Ca7 | Ca8 | Ca9 | Ca10 | Ca11 | Ca12 | Ca13 | Ca14 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
(A) Glandless cottonseed | |||||||||||||||
Vicilin GC72-A | 71 | 32.78 | 33.77 | 31.51 | 30.76 | 19.19 | 36.94 | 32.16 | 23.68 | 31.92 | 27.31 | 22.37 | 15.98 | 36.26 | 18.83 |
Vicilin C72 (G. hirsutum) | 70 | 26.01 | 21.71 | 21.89 | 24.49 | 38.38 | 22.72 | 23.28 | 25.64 | 24.53 | 28.88 | 31.18 | 17.77 | 20.88 | 27.95 |
Vicilin C72 | 70 | 23.26 | 20.22 | 20.38 | 21.55 | 31.98 | 22.72 | 22.45 | 25.64 | 24.21 | 22.59 | 29.46 | 0.00 | 19.12 | 0.00 |
Legumin A | 58 | 6.59 | 8.53 | 8.49 | 7.18 | 2.65 | 6.34 | 7.37 | 5.09 | 5.35 | 3.73 | 3.44 | 2.33 | 7.91 | 1.64 |
Vicilin-like antimicrobial peptides 2–1 | 62 | 3.85 | 4.82 | 5.09 | 4.05 | 2.96 | 4.79 | 3.35 | 7.63 | 3.62 | 2.16 | 2.58 | 5.03 | 3.96 | 2.54 |
Legumin B | 59 | 2.38 | 2.78 | 3.96 | 4.24 | 0.78 | 1.70 | 3.35 | 2.35 | 4.09 | 3.73 | 3.23 | 3.05 | 1.32 | 0.75 |
2S albumin storage protein | 16 | 0.18 | 1.11 | 0.94 | 2.76 | 0.78 | 0.31 | 1.68 | 0.59 | 0.47 | 3.73 | 0.65 | 2.51 | 4.40 | 5.68 |
Vicilin-like antimicrobial peptides 2–2 | 55 | 0.37 | 1.11 | 0.94 | 0.55 | 0.00 | 0.00 | 0.50 | 0.78 | 0.16 | 0.59 | 0.22 | 2.33 | 0.22 | 0.90 |
Asparticase# | 60 | 0.37 | 0.00 | 0.00 | 0.37 | 0.47 | 1.08 | 0.34 | 0.20 | 0.31 | 0.00 | 0.43 | 1.26 | 0.00 | 0.30 |
60S ribosomal L10a-1-like protein# | 25 | 0.55 | 0.93 | 0.57 | 0.55 | 0.00 | 0.00 | 0.67 | 0.59 | 0.31 | 0.00 | 0.43 | 0.18 | 0.00 | 0.00 |
Top 10 total | 96.34 | 94.99 | 93.77 | 96.50 | 97.19 | 96.60 | 95.14 | 92.17 | 94.97 | 92.73 | 93.98 | 50.45 | 94.07 | 58.59 | |
MW, kDa | Ca1 | Ca2 | Ca3 | Ca4 | Ca5 | Ca6 | Ca7 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
(B) Glanded cottonseed4 | |||||||||||||||
Vicilin C72 (G. hirsutum) | 70 | 35.56 | 18.12 | 14.58 | 14.76 | 26.78 | 20.32 | 25.28 | |||||||
Vicilin C72 (G. arboretum) | 70 | 39.87 | 18.49 | 13.54 | 14.51 | 23.45 | 20.55 | 22.68 | |||||||
Vicilin GC72-A | 71 | 15.09 | 45.78 | 15.48 | 10.91 | 11.35 | 25.64 | 10.60 | |||||||
Legumin A | 58 | 2.80 | 8.56 | 38.39 | 26.70 | 11.80 | 15.94 | 27.51 | |||||||
Legumin B | 59 | 2.48 | 2.73 | 6.40 | 29.40 | 18.61 | 8.55 | 5.95 | |||||||
Vicilin-like antimicrobial peptides 2–1 | 62 | 1.08 | 1.86 | 3.42 | 0.26 | 1.06 | 1.39 | 2.04 | |||||||
Late embryogenesis abundant protein D-19 | 11 | 0.22 | 0.00 | 0.89 | 0.64 | 0.61 | 0.69 | 0.93 | |||||||
ATP synthase subunit beta # | 60 | 0.43 | 0.25 | 0.74 | 0.13 | 0.76 | 0.46 | 0.37 | |||||||
Heat shock protein 70 | 71 | 0.00 | 0.12 | 1.04 | 0.00 | 1.06 | 0.00 | 0.37 | |||||||
Elongation factor 1-alpha | 49 | 0.65 | 0.25 | 0.60 | 0.13 | 0.00 | 0.23 | 0.56 | |||||||
Top 10 total | 98.17 | 96.16 | 95.09 | 97.44 | 95.46 | 93.77 | 96.28 | ||||||||
