Figure 3

(A) comparison between typical transfer curves obtained for thrombin-incubated and non-incubated gate electrodes (TBA15-functionalized AuNPs-PMLG, blue line; unfolded TBA15-functionalized AuNPs-PMLG, concentration 1 μM -green line; bare PMLG, red line, and AuNPs-PMLG, black line. In the inset: proposed detection method) and (B) related gate currents. Relative peaks are indicated with (2) for the AuNPs-PMLG, (3) for TBA15-functionalized AuNPs-PMLG and (4) for TBA15-functonalized AuNPs-PMLG gate electrode; (C) typical device response to different concentrations of thrombin (\(C_{Thr}\)) described by introducing the \(\Delta ratio\) parameter; (D) selectivity test: the signal recorded in the presence of higher concentration of BSA (1 mM) is compared with the one obtained in presence of very low thrombin concentration (100 pM).