Figure 4
From: Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration
Experimental set-up for the analysis of Rbm24 functions in muscle regeneration. (a) Western blot analysis of Rbm24 protein levels in isolated satellite cells from indicated conditions. Tubulin served as a loading control. Full-length blots are presented in Supplementary Fig. S3. (b) Quantification of western blot results followed by ANOVA analysis shows the significant effect of Rbm24 knockdown. Rbm24 protein level in untransfected satellite cells is set to 1 as a reference, after normalization to tubulin. Data are the mean ± s.e.m. from three independent experiments. ANOVA F (df 2.6) = 6.182, p < 0.05; *p < 0.05 by Tukey’s HSD test. (c–e) Co-transfection of pEGFP-N1 and pCS2-RFP plasmids mixed at a 1:10 ratio. DAPI was used to stain nuclei. Note that all GFP-positive cells also expressed RFP. From this result, it can be extrapolated that, when pEGFP-N1 and shScramble or shRbm24 plasmids are used at the same ratio, GFP-labeled cells along with a proportion of unlabeled cells should be targeted by shRbm24. (f) Quantification of GFP-positive or RFP-positive cells. Data are the mean ± s.e.m. from three independent experiments. *p < 0.05 by Tukey’s HSD test. (g) Quantification of the overlapping expression of GFP with RFP in transfected satellite cells. Data are the mean ± s.e.m. from three independent experiments.
