Figure 4 | Scientific Reports

Figure 4

From: PKR activity modulation by phosphomimetic mutations of serine residues located three aminoacids upstream of double-stranded RNA binding motifs

Figure 4

dsRNA binding contributes to spontaneous activation of S6A-S97A PKR mutant. (a) Schematic representation of the main PKR domains. The coordinates on the top are those of the four lysines mutated to alanines in order to generate a DRBM-dead PKR (PKR-4KA). (b) PKR-KO cells were transfected with plasmids expressing PKR mutants and fixed 48 h post-transfection for eIF3 and PKR detection by immunofluorescence. Graphs (generated with Microsoft Excel 2011) show the mean and SEM of the percentages of stress granule-positive cells counted among PKR+ cells (n = 4). 1-way ANOVA (Graphpad Prism 7) compared each PKR condition to the S6A-S97A PKR mutant condition.

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