Figure 3

Nano CaCO₃ effects on MDA-MB-231 cell growth. (A) Representative images of the MDA-MB-231 cells loaded in fibrin gels. Images of the start (top) and end (bottom) of the experiment with fibrin gels containing 1.6 mg mL⁻¹ concentration of CaCO₃ nanoparticles is shown. Scale bar = 200 µm. (B) Embedding CaCO₃ nanoparticles inside fibrin. After embedding CaCO₃ nanoparticles inside our fibrin gel, the quantification of tumor growth showed significantly more growth under the control conditions. **** p < 0.0001. (C) Representative images of MDA-MB-231 cells expressing RFP grown in varying concentrations of CaCO₃ nanoparticles. Images of the tissue chambers right after loading are shown in the top row, and images at the end of the experiment are shown in the bottom row. Scale bar = 200 µm. (D) Quantification of the effects of CaCO₃ nanoparticles at varying concentrations. At 0.8 mg mL⁻¹, tumor growth was significantly inhibited compared to the control condition. * p < 0.05.