Figure 4 | Scientific Reports

Figure 4

From: Calcium carbonate nanoparticles stimulate cancer cell reprogramming to suppress tumor growth and invasion in an organ-on-a-chip system

Figure 4

Nano CaCO3 effects on pH. (A) After the cells were seeded in 24 well plates and received media with or without CaCO3 nanoparticles, the media was collected and a pH probe was used to make measurements. The control group (red) had significantly lower pH than the experimental group (blue). (B) A pHrodo Green AM Intracellular pH Indicator dye was used compare the intracellular pH of the tumor cells with or without CaCO3 nanoparticles. (C) Higher fluorescent intensity indicates lower intracellular pH, and a Calibration Buffer Kit was used to correlate fluorescent intensity with intracellular pH. (D) The average intracellular pH of the MDA-MB-231 cells without nanoparticles was significantly higher than those grown with nanoparticles indicating a more alkaline intracellular pH. Scale bar = 100 µm, * p < 0.05.

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