Figure 3
Mild to severe glaucomatous RGC soma and axon degeneration in HF, LF and ND eyes at 8wpi. (A) Representative OCT images of mouse retinal area surrounding the ON head. GCC: ganglion cell complex, including RNFL, GCL and IPL layers; indicated by double end arrows. (B) Quantification of GCC thickness, represented as percentage of GCC thickness in the SO eyes, compared to the CL eyes. Control (CON): n = 17; HF: n = 30; LF: n = 30; ND: n = 14. **: p < 0.01, ****: p < 0.0001, one-way ANOVA with Tukey’s multiple comparison test. (C) Upper panel, confocal images of wholemount retinas showing surviving RBPMS-positive (red) RGCs of naïve, HF, LF and ND eyes at 8wpi. Scale bar, 100 µm. Middle panel, confocal images of a portion of the peripheral retina showing surviving RBPMS-positive (red) RGCs in the corresponding groups. Scale bar, 20 µm. Lower panel, light microscope images of semi-thin transverse sections of ON stained with PPD in the corresponding groups. Scale bar, 10 µm. (D,E) Quantification of surviving RGCs in the peripheral retina and surviving axons in ON of the corresponding groups at 8wpi, represented as percentage of SO eyes compared to CL eyes. (F) Quantification of P1-N2 amplitude, represented as percentage of P1-N2 amplitude in the SO eyes, compared to CL eyes. Data are presented as means ± s.e.m. Control (CON): n = 12; HF: n = 26; LF: n = 30; ND: n = 27, * p < 0.05, ** p < 0.01, ***: p < 0.001, ****: p < 0.0001; one-way ANOVA with Tukey’s multiple comparison test.
