Figure 1 | Scientific Reports

Figure 1

From: Phagocytic and pinocytic uptake of cholesterol in Tetrahymena thermophila impact differently on gene regulation for sterol homeostasis

Figure 1

Kinetics of radiolabeled cholesterol incorporation in T. thermophila wild-type (CU428) and phagocytosis-deficient (II8G-IA) strains. Exponentially growing cells cultured at 37 °C were incubated with 26 μM, 1.54 mCi/mol [14C] − cholesterol. Samples of identical volume were collected at various time points and radioactivity was measured on cell pellets as disintegrations per minute (DPM). Cytochalasin D (CytD, 2 μM final concentration) was added 30 min before cholesterol. Data are expressed as mean and range of two replicates from a representative experiment, repeated three times with similar results.

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