Figure 2 | Scientific Reports

Figure 2

From: Phagocytic and pinocytic uptake of cholesterol in Tetrahymena thermophila impact differently on gene regulation for sterol homeostasis

Figure 2

Effect of cholesterol on gene expression depends on the uptake route. Cells of wild-type (CU428) and phagocytosis-deficient (II8G-IA) strains were cultured at 37 °C (a) or 30 °C (b), and treated with 26 μM cholesterol 1 h prior to RNA extraction. Expression of representative genes involved in sterol and lipid metabolism which are differentially regulated was analyzed by RT-qPCR. Induced genes: DES22B, putative sterol C22 desaturase B; MLup, up-regulated ML domain protein; Δ6DES, delta-6 fatty acid desaturase. Repressed genes: SQS, squalene synthase; MLdw, down-regulated ML domain protein. Data are expressed as mean ± SD (n = 3) log2 fold change relative to untreated cells of the respective strain. *p < 0.05 vs. untreated cells, Student’s t test.

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