Figure 3 | Scientific Reports

Figure 3

From: Phagocytic and pinocytic uptake of cholesterol in Tetrahymena thermophila impact differently on gene regulation for sterol homeostasis

Figure 3

Effects of Cytochalasin D on the expression of cholesterol-regulated genes in wild-type (CU428) and phagocytosis-deficient (II8G-IA) strains. Cultures at exponential growth phase were incubated with 2 μM Cytochalasin D (CytD) or vehicle for 30 min prior to the addition of 26 μM cholesterol. Total RNA was extracted after 1 h of treatment with cholesterol and gene expression was analyzed by RT-qPCR. Data are expressed as mean ± SD (n = 3) log2 fold change relative to untreated cells of the respective strain. * p < 0.05 vs. untreated cells; # p < 0.05; ns, not significant difference; One-way ANOVA with Tukey post hoc test.

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