Figure 4
Cholesterol concentration-dependent response curves for the expression of DES22B and SQS in wild-type cells. Cultures were supplemented with different concentrations of cholesterol between 0.25 to 25 µM and RNA was extracted after 1 h for RT-qPCR analysis. Data represent the percentage of the maximum log2 fold change relative to untreated cells and are shown as mean ± SD (n = 3). Note that although SQS expression is repressed by cholesterol, the response here is plotted as positive values in order to allow a better visualization and comparison of the curves. The grey line indicates the threshold above which expression changes are considered significative.
