Figure 7
U18666A delays changes in the expression of cholesterol-regulated genes. Time course of the expression of DES22B and SQS in wild-type (a) or phagocytosis-deficient (b) cells. Cultures at exponential growth phase were incubated with 2 μM U18666A or vehicle for 15 min prior to the addition of 26 μM cholesterol. Total RNA was extracted at different times after treatment with cholesterol and gene expression was analyzed by RT-qPCR. Data are expressed as mean ± SD (n = 3) log2 fold change relative to untreated cells (before addition of U18666A or cholesterol) of the respective strain. Statistical analysis was performed by repeated measures two-way ANOVA with Bonferroni post hoc test to compare treatments at each time; # p < 0.05; ns, not significant difference.
