Figure 8
Effect of Brefeldin A on the expression of cholesterol-regulated genes. Time course of the expression of DES22B and SQS in wild-type cells. Cultures at exponential growth phase were incubated with 36 μM Brefeldin A (BFA) or vehicle for 2 h prior to the addition of 26 μM cholesterol. Total RNA was extracted at different times after treatment with cholesterol and gene expression was analyzed by RT-qPCR. Data are expressed as mean ± SD (n = 3) log2 fold change relative to cells before the addition of cholesterol. Statistical analysis was performed by repeated measures two-way ANOVA with Bonferroni post hoc test to compare treatments at each time; ns, not significant difference.
