Figure 5
From: Identification of candidate PAX2-regulated genes implicated in human kidney development
Identification of candidate PAX2-regulated kidney development genes by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). (a) mRNA samples were collected on iPSCs and sorting nephron progenitor cells using the surface markers INTEGRINα8+ PDGFRα−. (b) mRNA expression levels of PAX2 and candidate genes on iPSCs and sorted nephron progenitor cells using the surface markers INTEGRINα8+ PDGFRα−. (c) mRNA samples were collected on iPSCs, day 9, day 11, and day 14. (d) mRNA expression levels of PAX2 and candidate genes during induction of control and RCS-iPSCs to nephron progenitor cells in vitro. Data are expressed as mean ± SEM, n = 5 per group. Comparison of gene expressions between days 11 and 14 in healthy control samples was evaluated using Student’s t-test. Comparison of gene expressions between healthy control and RCS samples on day 14 was evaluated using Student’s t-test. *Statistically significant, **P < 0.01; ***P < 0.001; ****P < 0.0001. n.s. not significant.
