Figure 4

Candidate expression of genes involved in BCAA transport (a) and BCAA catabolism (b) for control (black), BCH (pink), hypoxia (red) and BCH + hypoxia (gold) groups. (a) Hypoxia was associated with an increase in the expression of SLC1A5 (p = 1.53E−7, f = 42.70) but not SLC38A2 (p = 0.017, f = 6.29). There were no main effects of BCH (SLC1A5-p = 0.30, f = 1.09; SLC38A2-p = 0.25, f = 1.37) or interaction effects (SLC1A5-p = 0.47, f = 0.54; SLC38A2-p = 0.61, f = 0.26). (b) There were no significant main effects of hypoxia or BCH on the expression of Acadsb (hypoxia-p = 0.01, f = 6.95; BCH-p = 0.53, f = 0.39), Acat1 (hypoxia-p = 0.30, f = 1.13; BCHp = 0.40, f = 0.74) or Bcat1 (hypoxia-p = 0.04, f = 4.76; BCH-p = 0.75, f = 0.10) and no interaction effects (Acadsb-p = 0.89, f = 0.02; Acat1-p = 0.43, f = 0.62; Bcat1-p = 0.65, f = 0.21). p < 0.004 was the adjusted threshold for statistical significance, *** indicates p < 0.001. Statistical comparisons were made using a 2-way ANOVA using a Bonferroni adjusted p-value. Expression was normalised to TBP. Error bars indicate standard error.