Figure 3
Sensitivity and specificity of the ELISA using full-length E protein. (A) Reactivity of WNV-infected (n = 3), JEV-infected (n = 3), and TBEV-infected (n = 3) mouse sera in the ELISA. Negative sera (mock; n = 3) were used as controls. Standardized serum from a WNV-, JEV-, or TBEV-infected mouse was serially diluted as described. The results were recorded as P/N ratios. The difference between the WNV-infected mouse serum and JEV-infected, TBEV-infected mouse serum, or mock was significant (**p < 0.01) according to the Tukey–Kramer test. (B,C) Correlation between the ELISA signal (P/N ratio) and results from the IFA or NT. Serum samples from mice infected with WNV were tested using ELISA and the results were compared to the titers estimated from the IFA (B) and NT (C). The strengths of the correlations (rs) were calculated as Spearman’s rank correlation coefficients (A, rs = 0.90; B, rs = 0.88). Red bars indicate approximate curves.
