Figure 4 | Scientific Reports

Figure 4

From: Dietary cis-9, trans-11-conjugated linoleic acid reduces amyloid β-protein accumulation and upregulates anti-inflammatory cytokines in an Alzheimer’s disease mouse model

Figure 4

The c-9, t-11-CLA diet increased the number of CD45+ and CD206+ microglia. (A) Double immunostaining of brain sections of c-9, t-11-CLA diet-fed and control diet-fed AD model mice with anti-CD45 and anti-IBA-1 (microglia marker) antibodies. A portion of IBA-1 (green)-positive cells were CD45 (red)-positive as shown by the yellow color (right merged panels). The arrows indicate the representative CD45 (red)-and IBA-1 (green)-positive cells as shown by yellow color (right merged panels). Small red dots (representatives marked with an asterisk) were nonspecifically stained as judged from the staining of the brain sections without the 1st antibody. Bar, 20 µm. WT: wild-type mice, AD: AD model mice. CLA + : c-9, t-11-CLA diet-fed AD mouse model; CLA − : control diet-fed AD model mice. (B) Histograms show the numbers of CD45-positive and IBA-1-positive signals (n = 6 mice for each group; five different fields per mouse were counted). **P < 0.01 (hippocampus, P = 0.01; cortex, P = 0.0097). (C) Double immunostaining of brain sections in hippocampus of c-9, t-11-CLA diet-fed and control diet-fed AD model mice with anti-CD206 and anti-IBA-1 (microglia marker) antibodies. The arrows indicate the representative CD206 (red)-and IBA-1 (green)-positive cells as shown by yellow color (right merged panels). Brain sections in the cortex was not clearly immunostained with anti-CD206 antibody (data not shown). (D) Histograms show the numbers of CD206-positive and IBA-1-positive signals (n = 6 mice for each group; five different fields per mouse were counted). (E) CD45+ and CD206+ microglia were co-localized with Aβ deposits. The brain sections in hippocampus of c-9, t-11-CLA diet-fed AD model mice were doubly immunostained with anti-CD45 (green) and anti-Aβ (red) antibodies (upper panels) or anti-CD206 and anti-Aβ (red) antibodies (lower panels). The arrow indicates the representative CD45 (green)- or CD206- and 6E10 (red)-positive cells as shown by yellow color (right merged panels). 6E10 was used for the anti-Aβ (red) antibody. The insets in right panels show the magnified pictures indicated by arrows. Bar, 20 µm.

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