Figure 5 | Scientific Reports

Figure 5

From: Microvesicles released from pneumolysin-stimulated lung epithelial cells carry mitochondrial cargo and suppress neutrophil oxidative burst

Figure 5

Pneumolysin (PLY) induces alveolar epithelial microvesicle (MV) release through pore formation and calcium influx. (A) A549 cells were treated with PLY or non-lytic (NL)-PLY (100 ng/ml) for 4 h and isolated MVs were analyzed by FACS. Depicted are representative dot blots of MVs after staining with annexin V. Positive events are indicated in the blue border gate. (B) A549 cells were pre-treated with BAPTA-AM (30 μΜ) for 1 h and then treated with PLY (100 ng/ml, 1 h). MVs were quantified by FACS after staining with annexin V. N = 3, *p < 0.001 vs Ctr, #p < 0.05 vs PLY (One-way Anova).

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