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Figure 1

From: Theileria equi claudin like apicomplexan microneme protein contains neutralization-sensitive epitopes and interacts with components of the equine erythrocyte membrane skeleton

Figure 1

T. equi CLAMP contains immunogenic epitopes, and the gene is transcribed and expressed by T. equi merozoites. (a) CLAMP’s transmembrane topology as predicted and annotated by Phobius and Protter algorithms, respectively. Peptides 1, 2 and 3 indicate the protein’s predicted immunogenic peptides. (b) A 1% agarose gel showing amplified clamp transcript at ~ 1226 bp. The T. equi equine merozoite antigen-1 (ema-1) transcript was amplified as a positive control, and rt- (negative control) represents amplified merozoites’ RNA without addition of reverse transcriptase. L indicates the Thermo Scientific GeneRuler 1 kb DNA Ladder. (c) Immunoblot showing CLAMP expressed by T. equi merozoites (test) at ~ 39 kDa. Probing of merozoites lysate with pre-immunization serum and the polyclonal anti-RAP-1a antibody were used as negative control (NC) and positive control (PC) respectively. L represents the ProteinSimple Wes Ladder.

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