Figure 2
From: Metastasis is altered through multiple processes regulated by the E2F1 transcription factor
Gene expression changes in E2F1−/− mouse tumors, and E2F1 low human breast cancer. (A) Two volcano plots show significant fold changes in genes from Neu and PyMT mouse tumors respectively. Fold change was determined by subtracting the E2F1 KO mean from the E2F1 WT mean for each gene. Fold change and p value cutoff for Neu tumors was .5, and .05 respectively. Fold change and P value for PyMT tumors was 1.0 and .001 respectively. (B) Diagram represents data processing steps for human TCGA data. A volcano plot shows significant fold change genes in E2F1 high versus E2F1 low human HER2+ve tumors. Fold change was determined by subtracting samples in the lowest E2F1 quartile mean from the highest E2F1 quartile mean for each gene. Fold change cutoff and p value for human tumors was 2.0, and 10e−60 respectively. (C) GSEA plots generated for E2F1 WT versus E2F1 KO tumors (Neu and PyMT combined) show enrichment of Nucleotide excision repair, and WNT signaling pathways in E2F1 KO tumors.
