Figure 2

The alternative text for this image may have been generated using AI.

source of calcium. Hepatocytes were treated with different blocking agents and chelators 30 min prior to cortisol addition. All results are shown as % of cortisol (100 ng/ml)-treated fura2 intensity, which is represented by the horizontal line at 100%. Representative fluorescence images taken at 1 min either with or without the addition of cortisol are shown above (ai–hi), while the corresponding quantification is shown below as a bar graph (a–h). Bar graph showing fura2 intensity ([Ca²⁺]i) changes at 60 s relative to cortisol treatment with (a) either control, EGTA or EGTA + Cortisol, (b) either control, BAPTA or BAPTA + Cortisol, (c) either control, Nifedipine (Nife) or Nife + Cortisol, (d) either control, cadmium (CAD) or CAD + Cortisol, (e) either control, U73122 or U73122 + Cortisol, (f) either control, xestospongin C (XeC) or XeC + Cortisol, (g) either control, H-89 or H-89 + Cortisol, (h) either control, ryanodine or ryanodine + Cortisol. All bars represent mean ± SEM (n = 5–6 independent fish; ~ 100 cells from each fish); bars with different letters are significantly different (one-way ANOVA; p < 0.05).