Figure 5 | Scientific Reports

Figure 5

From: Cells/colony motion of oral keratinocytes determined by non-invasive and quantitative measurement using optical flow predicts epithelial regenerative capacity

Figure 5

Images of pre-processing to calculate the MS by OF algorithm. (A) Representative original phase-contrast microscopic image of the p1 oral keratinocytes of the first frame of time-lapse microphotography. Cells are grown at approximately 50% confluence. (B) Illustration of edge detection for (A). Edges are detected by combining the horizontal and vertical derivatives using the square root of the sum of squares after 3 × 3 convolution kernels (Sobel filter) was applied to the image. (C) Illustration of the segmentation of cells/colonies by binarisation of (B). Black and white areas indicate background and cell area, respectively, by using the variational segmentation method of Chan and Vese. (D) Displacement vectors to be calculated by the OF algorithm in (A) are shown. By using the OpenCV library (ver.4.1), this full-screen image and the image at the next time step were used to calculate the MS as the mean value of the magnitude of vectors. The original video file is provided in Supplementary Video 6.

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