Figure 6 | Scientific Reports

Figure 6

From: Cells/colony motion of oral keratinocytes determined by non-invasive and quantitative measurement using optical flow predicts epithelial regenerative capacity

Figure 6

Histologic analysis of the EVPOME received metabolic challenges compared with the standard protocol. Representative histologic appearances of the EVPOMEs consisted of oral keratinocytes cultured under standard or four different metabolic challenge protocols in a 2D culture condition before transfer to a 3D culture condition. Oral keratinocytes grown in the standard protocol developed a well-differentiated, stratified epithelial layer on the scaffold (A,F). Compared with those histologic features, however, cells that received mild metabolic challenges, such as 5×PBS and 4dNoF, formed a thinner epithelial layer (B,D,G,I). Furthermore, cells that received severe metabolic challenges, such as 20×PBS and 7dNoF, generated a poorly differentiated epithelial layer (C,E,H,J). Additionally, some failed to generate a continuous epithelial layer. Haematoxylin and eosin staining. (n = 8 individuals) Original magnifications × 10 (A–E), × 40 (F–J). Scale bar = 100 μm.

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