Figure 7 | Scientific Reports

Figure 7

From: Cells/colony motion of oral keratinocytes determined by non-invasive and quantitative measurement using optical flow predicts epithelial regenerative capacity

Figure 7

Ki-67 immunohistochemical analysis of the EVPOME received metabolic challenges compared with the standard protocol. Representative images of Ki-67 immunohistochemistry of the EVPOMEs. Ki-67-positive cells are mainly present in the basal layer. The number of Ki-67 positive cells was remarkably fewer when cells received metabolic challenges before development of the EVPOMEs. (A) Standard protocol, (B) metabolic challenge of 5×PBS, (C) metabolic challenge of 20×PBS, (D) metabolic challenge of 4dNoF, (E) metabolic challenge of 7dNoF. Original magnification × 40. Scale bar = 100 μm.

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