Table 3 Predicted pathways and genes affected by aberrantly expressed miRNAs in TLE with onset in adulthood and infancy.

Predicted targets in infantile-onset epilepsy

Acute

TGF-β signalling

miR-205, -330-3p,-3473, -7a-2-3p

Smad1

SMAD family member 1

Smad5

SMAD family member 5

Smad7

SMAD family member 7

Bambi

BMP and activin membrane-bound inhibitor

Id2

Inhibitor of DNA binding 2, HLH protein

Zfyve16

Zinc finger FYVE-type containing protein 16

Ppp2ca

Serine/threonine-protein phosphatase 2CA catalytic subunit alpha isoform

Acvr1

Activin A receptor type 1

Smurf2

SMAD specific E3 ubiquitin protein ligase 2

Morphine addiction

miR-205, -221-3p, -330-3p

Gabra1

Gamma-aminobutyric acid type A receptor gamma 1 subunit

Gnb3

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3

Pde3b

Phosphodiesterase 3B

Long-term depression

miR-205, -221-3p, -330-3p,-7a-2-3p

Gnai2

Guanine nucleotide-binding protein G(s) subunit alpha

Itpr2

Inositol 1,4,5-trisphosphate receptor, type 1

Ppp2ca

Serine/threonine-protein phosphatase 2CA catalytic subunit alpha isoform

Hippo signalling

miR-205, -221-3p, -330-3p,-7a-2-3p

Smad1

SMAD family member 1

Smad7

SMAD family member 7

Ppp2ca

Serine/threonine-protein phosphatase 2CA catalytic subunit alpha isoform

Ywhag

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta

Id2

Inhibitor of DNA binding 2, HLH protein

Prkci

Protein kinase C, iota

Axin2

Axin 2

Tef7l2

Lymphoid enhancer binding factor 2

Dlg2

Discs large MAGUK scaffold protein 1

Wnt signalling

miR-205, -221-3p, -330-3p,-6215, -7a-2-3p

Bambi

BMP and activin membrane-bound inhibitor

Tbl1xr1

Transducin (beta)-like 1 X-linked

Nfatc3

Nuclear factor of activated T-cells 3

Ppp3r1

Protein phosphatase 3, regulatory subunit B, alpha

Prickle2

Prickle planar cell polarity protein 2

Cxxc4

CXXC finger protein 4

Axin2

Axin 2

Siaha

Siah E3 ubiquitin protein ligase 1

Sox17

Transcription factor SOX17

Tef7l2

Lymphoid enhancer binding factor 2

Nlk

Nemo like kinase

Glutamatergic synapse

miR-205, -221-3p, -330-3p,-6215

Gnb3

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3

Gnai2

G protein subunit alpha i2

Ppp3r1

Protein phosphatase 3, regulatory subunit B, alpha

Itpr2

Inositol 1,4,5-trisphosphate receptor, type 1

Homer2

Homer scaffolding protein 1

GABAergic synapse

miR -205, -221-3p, -330-3p

Gabra1

Gamma-aminobutyric acid type A receptor gamma 1 subunit

Gnb3

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3

Trak2

Trafficking kinesin protein 2

Chronic

Pentose phosphate pathway

miR-451-5p

Prps2

Phosphoribosyl pyrophosphate synthetase 2

Glutamate metabolism

miR-451-5p

Gfpt2

Glutamine fructose-6-phosphate transaminase 2

Sugar metabolism

miR-451-5p

Gfpt2

Glutamine fructose-6-phosphate transaminase 2

Biosynthesis of amino acids

miR-451-5p

Prps2

Phosphoribosyl pyrophosphate synthetase 2

Carbon metabolism

miR-451-5p

Prps2

Phosphoribosyl pyrophosphate synthetase 2

Predicted targets in adult-onset epilepsy

Acute

MAPK signalling

miR-132-3p, -142-3p, -142-5p, -155-5p, -212-3p, -361-3p

Cacnb4

Calcium voltage-gated channel auxiliary subunit beta 4

DUSP2 And 9

Dual specificity phosphatase 2 and 9

Fgf14

Fibroblast growth factor 4

Il1a

Interleukin 1 alpha

MAP3K3, 6 And 14

Mitogen activated protein kinase 3, 6 and 14

Mapkapk2

Mitogen-activated protein kinase-activated protein kinase 2

Mecom

Ecotropic virus integration site 1 protein

Nlk

nemo like kinase

Ptpn5

Protein tyrosine phosphatase, non-receptor type 5

Rap1b

Ras-related protein Rap-1B

Rasa1

RAS P21 Protein Activator 1

Rasgrf1

RAS protein-specific guanine nucleotide-releasing factor 2

Rela

RELA proto-oncogene, NF-kB subunit

RPS6KA3 And 4

Ribosomal protein S6 kinase A3 and 4

RRAS Adn 2

Ras-related protein R-Ras

Sos1

SOS Ras/Rac guanine nucleotide exchange factor 1

Srf

Serum response factor

Tab2

TGF-beta activated kinase 1/MAP3K7 binding protein 2

Tgfbr1

Transforming growth factor, beta receptor 1

Ras signalling

miR-132-3p, -142-3p, -142-5p, -155-5p, -212-3p, -361-3p

Bcl2l1

Bcl-2-like 1 (apoptosis regulator Bcl-X)

Csf1r

Colony stimulating factor 1 receptor

ETS1 And 2

ETS proto-oncogene 1 and 2, transcription factor

Foxo4

Forkhead box protein O4

Gab1

GRB2-associated binding protein 1

Grin2a

Glutamate Ionotropic Receptor NMDA Type Subunit 2A

Kitlg

KIT ligand

Rap1b

Ras-related protein Rap-1B

Rasa1

RAS P21 Protein Activator 1

Rasgrf1

RAS protein-specific guanine nucleotide-releasing factor 1

Rela

RELA proto-oncogene, NF-kB subunit

Rgl2

Ral guanine nucleotide dissociation stimulator-like 2

RRAS And 2

Ras-related protein R-Ras

Latent

Mucin type O-glycan biosynthesis

miR-185-5p, -7b

Galnt3

Polypeptide N-acetylgalactosaminyltransferase3

Galnt5

Polypeptide N-acetylgalactosaminyltransferase5

Synaptic vesicle cycle

miR-185-5p, -7b

Syt1

Synaptotagmin 1, isoform A

Snap25

Synaptosomal-associated protein 25

Slc18a2

Solute carrier family 18 member 2

Atp6v1e1

ATPase H + transporting V1 subunit E1

Chronic

Amphetamine addiction

miR-142-5p,-146a-5p, -221-3p

Ddc

Dopa decarboxylase

Fos

FBJ osteosarcoma oncogene

Ppp3r1

Protein phosphatase 3, regulatory subunit B, alpha

Slc18a2

Solute carrier family 18 (vesicular amine transporter), member 2

Cocaine addiction

miR-142-5p,-146a-5p, -221-3p

Dlg4

Discs large MAGUK scaffold protein 4

Gnai2

G protein subunit alpha i2

Ddc

Dopa decarboxylase

Nfkb1

Nuclear Factor Kappa B Subunit 1

Slc18a2

Solute carrier family 18 (vesicular amine transporter), member 2

GABAergic synapse

miR-146a-5p, -205, -221-3p

Gabra1

Gamma-aminobutyric acid type A receptor gamma 1 subunit

Gnb3

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3

Trak2

Trafficking kinesin protein 2

Scl38a5

Solute carrier family 38 (sodium-coupled neutral amino acid transporter), member 5

Hippo signalling

miR-205, -221-3p, -330-3p,-142-5p, -146a-5p

Smad1

SMAD family member 1

Smad4

SMAD family member 4

Smad7

SMAD family member 7

Ywhag

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta

Id2

Inhibitor of DNA binding 2, HLH protein

Axin2

Axin 2

Tef7l2

Lymphoid enhancer binding factor 1

DLG2 And 4

Discs large MAGUK scaffold protein 2 and 4

Morphine addiction

miR-142-5p, -205, -221-3p

Gabra1

Gamma-aminobutyric acid type A receptor gamma 1 subunit

Gnb3

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3

Pde3b

Phosphodiesterase 3B

NF-κB signalling

miR-146a-5p, -221-3p

Traf6

TNF receptor associated factor 6

Map3k14

Mitogen-activated protein kinase kinase 14

Nfkb1

Nuclear Factor Kappa B Subunit 1

Irak1

Interleukin-1 receptor-associated kinase 4

TGF-β signalling

miR-205, -142-5p, -146a-5p

Smad1

SMAD family member 1

Smad4

SMAD family member 4

Smad7

SMAD family member 7

Bambi

BMP and activin membrane-bound inhibitor

Id2

Inhibitor of DNA binding 2, HLH protein

Zfyve16

Zinc finger FYVE-type containing protein 16

Ubiquitin mediated proteolysis

miR-205, -221-3p,-142-5p, -146a-5p

Herc3

E3 ubiquitin-protein ligase HERC3

Mgrn1

E3 ubiquitin-protein ligase MGRN1

Siah1a

Siah E3 ubiquitin protein ligase 1

Traf6

TNF receptor associated factor 6

UBE2a,2e3,3A, 4A

Ubiquitin-conjugating enzyme E2A, 2E3, 3A, 4A

Uba 6

Ubiquitin-like modifier activating enzyme 6

Wwp1

WW domain containing E3 ubiquitin protein ligase 1

Wnt signalling

miR-205, -221-3p, -330-3p,-6215, -7a-2-3p

Bambi

BMP and activin membrane-bound inhibitor

Tbl1xr1

Transducin (beta)-like 1 X-linked

Nfatc3

Nuclear factor of activated T-cells 3

Ppp3r1

Protein phosphatase 3, regulatory subunit B, alpha

Cxxc4

CXXC finger protein 4

Axin2

Axin 2

Siah1a

Siah E3 ubiquitin protein ligase 1

Nlk

Nemo like kinase

Vangl1

VANGL planar cell polarity protein 2

Fzd1

Frizzled 1/7