Figure 4 | Scientific Reports

Figure 4

From: Cholinergic stimulation with pyridostigmine modulates a heart-spleen axis after acute myocardial infarction in spontaneous hypertensive rats

Figure 4

CD68+ cell count (M1 macrophages) (A) Photomicrographs showing the M1 macrophages within the infarcted zone from the left ventricle (magnification 400×) and (B) bar graphs of CD68+ positive cells in each group was compared. Fifteen microscopic fields of the infarcted and peri-infarcted zones were analyzed (5–6 animals per group). *P < 0.05; §P < 0.05 statistically significant. §AMI versus Sham; *AMI + PY vs Sham. (Immunohistochemistry, diaminobenzidine: scale bar equals 50 µm). Values expressed as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by a multiple comparisons test Tukey’s was performed using GraphPad Prism version 9.0.1. www.graphpad.com.

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