Figure 6

Correlation of DEGs and DMGs in the combination treatment group. (a) Venn diagram representing unique and overlapping differentially expressed genes (DEGs) and differentially methylated genes (DMGs) in the combination (BSp + GTPs) treatment group. (b) Scatter plot for 39 genes which are differentially expressed and methylated. The y-axis represents the methylation difference across 39 genes (dots in red color) and x-axis represents log₁₀FC. Out of 39 transcripts, 2 genes (Tmem132d and Pdx1) were up-regulated and hypomethylated and 3 genes (Ndufa1, Rpl13 and Get4) were downregulated and hypermethylated. (c) Heatmap representing overlapping 5 up- and downregulated genes in the combination (BSp + GTPs) treatment group based on q value. Each row corresponds to differentially expressed and differentially methylated transcripts and each column represents biological replicates in control (N = 5) and the combination (N = 5) treatment group. Blue color denotes lower expression levels and red color denotes higher expression levels.