Figure 1
From: Differential accumulation of tau pathology between reciprocal F1 hybrids of rTg4510 mice
Abnormally phosphorylated tau and Gallyas-positive structures in the brains of rTg4510 mice at 6 months of age. (A) Representative photographs of the immunohistochemical results for human tau (clone HT7), phosphorylated tau (clone AT8), and Gallyas silver staining. Scale bar: 1 mm. (B–E) Quantitative analysis of the AT8-immunoreactive area and Gallyas-positive stained area. Two-way ANOVA (genetic background × sex) revealed a main effect of the genetic background, but no effect of sex, in the AT8-immunoreactive area (B) and Gallyas-positive stained area (D) of the cerebral cortex (Supplementary Table S1). The Mann–Whitney U test revealed significantly higher levels of AT8-immunoreactivity (C) and Gallyas-positive staining (E) in samples obtained from the rTg4510_FxC mice, as compared with the rTg4510_CxF mice. The rTg4510_CxF and rTg4510_FxC groups contained six males and six females each (n = 12/group). Data are presented as the mean ± SEM. ***p < 0.001 (Mann–Whitney U test).
