Figure 6
From: Differential accumulation of tau pathology between reciprocal F1 hybrids of rTg4510 mice
The level of tau accumulation and phosphorylation in MID1-knockdown cells. HEK293T cells stably expressing human tau with the P301L mutation were treated with negative control siRNA (NC) and MID1 siRNA (KD) for 48 h. (A) MID1 mRNA levels in NC-treated and MID1-knockdown cells. (B) Western blots of HT7, AT8, AT180, AT270, and β-actin in the TBS-soluble fractions. Arrows and arrowheads indicate bands at 55 and 64 kDa, respectively. Full-length blots are presented in Supplementary Figure S13. (C–I) Densitometric analysis. Data are presented as the mean ± SEM of three independent experiments. *p < 0.05 (Student’s t-test).
