Figure 2

Chromatograms of steroids using a P(NIPAAm-co-BMA) hydrogel-modified bead column. (A) 7-nm pore-diameter beads, (B) 12-nm pore-diameter beads, and (C) 30-nm pore-diameter beads as base materials. The mobile phase was pure water, and the elution of the analytes was monitored at 254 nm at a mobile-phase flow rate of 1.0 min/L. Peak 1 = hydrocortisone, peak 2 = prednisolone, peak 3 = dexamethasone, peak 4 = hydrocortisone acetate, and peak 5 = testosterone.