Figure 2

Co-registered morpho-molecular information of OCT and Raman images on a collagen rich bladder biopsy. Molecular Raman enface maps for epithelial (a), collagen (b) and lipid (c) content and enface OCT maximum intensity projection of ten slices from a depth of 120–150 µm (d). The Raman maps indicate a dominant and nearly homogenous collagen signal over the entire biopsy (b), whereas the lipid content and epithelial signal is not very pronounced (a,c). The molecular information corresponds well to the very bright OCT signal. Maximum intensity projections of 10 cross-sectional OCT B-scans are indicated for positions in the biopsy by dashed and dotted lines in the enface image (e,f), respectively. The co-registered molecular information for the three components (E: Epithelium, C: Collagen, L: Lipid) are plotted above. It can be seen that neither B-scans in (e) nor (f) do exhibit an epithelium layer, however, show features with a very bright appearance. For comparison, an H&E image of the respective biopsy is displayed in (g). In the H&E slice (g), only lamina propria tissue is present, green arrows. The whole section does not exhibit any epithelial cell layer. A region of edema within the lamina propria is identified. Further, two representative fibroblasts, building up the extracellular matrix constructed mainly of collagen fibers are pinpointed. Scale bars: 250 µm.