Figure 7
Localisation of miR-21-5p, miR-221-3p, and miR-222-3p by Laser Capture Microdissection analysis. (A–C) RT-qPCR for selected microRNAs in sham (n = 8), 45 min bilateral IRI (n = 9), localised pulsatile IPC prior to IRI (n = 8), and localised pulsatile IPC alone (n = 8), rats at 48 h after reperfusion (A) miR-21-5p, (B) miR-221-3p, (C) miR-222-3p (D–F) MicroRNA expression in nephron sub-compartments. Laser Capture Microdissection (LCM) of: Glomerulus, G; Proximal Convoluted Tubule, PCT; Distal Convoluted Tubule, DCT; and Vessel, V using cortical sections from Sham, IRI, IPC prior to IRI, and IPC alone (n = 3 animals per group). (D) miR-21-5p, (E) miR-221-3p, (F) miR-222-3p. Expression is normalised to miR-16-5p and plotted as mean ± SEM. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001. Statistically significant differences between groups have been indicated.
