Table 1 Screening of the selected individual CAZymes for Laminaria digitata cell wall disruption.
ID | Name | Category | EC number | Main substrate | Released reducing sugars scale | Decreased fluorescence intensity scale |
|---|---|---|---|---|---|---|
6 | Cellulase (Cel73;Cell73) | Cellulases | 3.2.1.4 | 1,3–1,4-β-glucans and soluble 1,4-β-glucans | − (0.26 g/L) | + (14.6%) |
18 | Laminarinase A (LamA) | 1,3-β-Glucanases | 3.2.1.-/3.2.1.39 | Laminarin (1,3–1,4/1,6-β-glucans) | + + (5.16 g/L) | + + + + (40.1%) |
20 | Endo-guluronate lyase (AlyA1;zobellia_1182) | Poly-α-guluronate lyases | 4.2.2.11 | Sodium alginate/β-elimination reaction | + + + + (6.63 g/L) | + + + + (49.8%) |
21 | β-1,3–1,4-glucanase P2 (LicP;GluB) | 1,3–1,4-β-Glucanases | 3.2.1.73 | 1,3–1,4-β-glucans | + + (5.10 g/L) | + + + + (51.6%) |
22 | Alginate lyase / poly-β-mannuronate (Sde_2547) | Alginate lyases | 4.2.2.3 | Alginates and oligoalginates | + + + + (6.78*-7.11** g/L) | + + + + (39.1**—46.5* %) |
28 | Cellobiohydrolase (CbhA; Cthe_0413) | Cellobiohydrolases | 3.2.1.-/3.2.1.91 | Amorphous and crystalline cellulose | + + + (5.42 g/L) | + + + + (57.0%) |
29 | Lytic transglycosylase A (MltA; Mlt;b2813) | Murein lyase/Exomuramidase | 4.2.2.n1 | Murein glycan strands and insoluble, high-molecular weight murein sacculi | + + (4.42 g/L) | + + + + (42.9%) |
46 | α-L-fucosidase C(AlfC; LCABL_29340 possible fragment) | Fucosidases | 3.2.1.51 F1-6Gn | p-nitrophenyl-α-L-fucopyranoside | − (0.65 g/L) | + + (22.3%) |
