Table 4 Content (mg/g alga) of total proteins, chlorophylls, carotenoids, fucoxanthins and fatty acids of the supernatant and residue fractions derived from the incubation of Laminaria digitata with control and alginate lyase (AL; ID 22) treatment.

From: An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall

	Supernatant				Residue
	Control	AL	SEM	p-value	Control	AL	SEM	p-value
Total protein	39.9	31.1	5.14	0.289	91.7	114.3	8.72	0.167
Chlorophyll a	0.013*	0.014	0.0019	0.643	0.124**	0.119	0.0028	0.274
Chlorophyll b	0.015*	0.018	0.0032	0.590	0.006**	0.005	0.0028	0.874
Total chlorophylls	0.028*	0.032	0.0051	0.612	0.130**	0.124	0.0050	0.443
Total carotenoids	0.024*	0.031	0.0053	0.413	0.034**	0.029	0.0015	0.071
Total chlorophylls + Total carotenoids	0.053*	0.063	0.0083	0.418	0.164**	0.153	0.0058	0.248
Total pheophytins	0.121*	0.140	0.0253	0.630	0.552**	0.534	0.0248	0.641
Fucoxanthins	0.021*	0.021	0.0011	0.842	0.016**	0.013	0.0010	0.084
Total fatty acids	1.30^b	4.22^a	0.233	0.001	1.34	1.82	0.432	0.516
Fatty acid composition (% total fatty acids)
12:0	0.61^a	0.33^b	0.032	0.004	0.58	0.56	0.040	0.798
14:0	2.24^a	1.27^b	0.136	0.007	3.01	3.88	0.880	0.555
15:0	0.76	0.44	0.102	0.090	0.72	0.55	0.230	0.652
16:0	35.45^a	22.18^b	0.432	< 0.001	36.31	31.59	1.772	0.200
16:1c9	0.75^b	1.13^a	0.082	0.029	1.54	1.80	0.240	0.521
17:0	3.32	2.50	0.354	0.179	3.71	2.47	0.901	0.433
18:0	34.21^a	28.27^b	1.176	0.023	25.60	23.51	2.242	0.578
18:1c9	10.16^b	31.94^a	1.388	< 0.001	13.31	18.26	2.752	0.331
18:1c11	1.07^b	2.29^a	0.230	0.020	1.14	1.46	0.339	0.579
18:2n-6	2.08	1.35	0.190	0.053	2.63	2.69	0.479	0.943
18:3n-3	0.30	0.26	0.112	0.845	1.04	1.30	0.550	0.772
20:0	1.74^a	0.62^b	0.104	0.002	1.48	1.33	0.149	0.534
20:1c11	0.21^b	0.89^a	0.154	0.036	0.54	1.04	0.119	0.096
20:4n-6	0.18	0.17	0.130	0.965	2.54	2.01	0.710	0.655
20:5n-3	0.34	0.24	0.248	0.776	1.90	1.66	0.468	0.752
21:0	3.15	3.09	1.667	0.980	1.38	2.48	1.029	0.527
22:0	1.59^a	0.51^b	0.259	0.043	1.08	1.15	0.296	0.874
22:1	1.54	0.65	0.250	0.065	1.20	1.03	0.238	0.669
Others	0.29^b	1.86^a	0.269	0.015	0.31	1.24	0.250	0.119
∑ SFA	83.07^a	59.21^b	1.801	0.001	73.87^a	67.53^b	0.893	0.037
∑ MUFA	13.73^b	36.90^a	1.442	< 0.001	17.71	23.58	2.603	0.252
∑ PUFA	2.91	2.02	0.365	0.163	8.11	7.66	2.204	0.899
∑ n-3 PUFA	0.64	0.50	0.287	0.748	2.94	2.96	1.017	0.991
∑ n-6 PUFA	2.26^a	1.52^b	0.172	0.038	5.17	4.70	1.188	0.807

Two mL of macroalgae suspension was incubated with alginate lyase at a final concentration of 20 mg/L. The control assay took the same amount of PBS. Incubations were done overnight at 37 ºC and 160 rpm. After incubations, supernatant and residue fractions were separated by centrifugation. Only fatty acids whose percentage was > 0.5% are presented. * Values measured in phosphate buffered saline (PBS); ** Values measured after extraction with acetone.

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Table 4 Content (mg/g alga) of total proteins, chlorophylls, carotenoids, fucoxanthins and fatty acids of the supernatant and residue fractions derived from the incubation of Laminaria digitata with control and alginate lyase (AL; ID 22) treatment.

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