Table 1 Orthogonal experiment (L9(34)) analyzing the effects of different factors on callus induction of P. grandiflorus.
Factors | Inoculation Num (non-contaminated) | Calli Num | Induction rate (%) | ||||
|---|---|---|---|---|---|---|---|
Media (A) | Explants (B) | NAA mg/L (C) | 6-BA mg/L (D) | ||||
1 | B5 | Leaves | 0.1 | 0.5 | 56 | 4 | 7.14 |
2 | B5 | Stems with nods | 0.2 | 1.0 | 58 | 22 | 37.93 |
3 | B5 | Stems | 1.0 | 2.0 | 57 | 8 | 14.04 |
4 | MS | Leaves | 0.2 | 2.0 | 53 | 6 | 11.32 |
5 | MS | Stems with nods | 1.0 | 0.5 | 57 | 57 | 100.00 |
6 | MS | stems | 0.1 | 1.0 | 54 | 2 | 3.70 |
7 | WPM | leaves | 1.0 | 1.0 | 55 | 0 | 0.00 |
8 | WPM | Stems with nods | 0.1 | 2.0 | 58 | 23 | 39.66 |
9 | WPM | stems | 0.2 | 0.5 | 56 | 4 | 7.14 |
k1 | 59.11 | 18.46 | 50.50 | 114.28 | |||
k2 | 115.02 | 177.59 | 56.39 | 41.63 | |||
k3 | 46.80 | 24.88 | 114.04 | 65.02 | |||
K1 | 19.70 | 6.15 | 16.83 | 38.09 | |||
K2 | 38.34 | 59.20 | 18.80 | 13.88 | |||
K3 | 15.60 | 8.29 | 38.01 | 21.67 | |||
R | 22.74 | 53.05 | 21.18 | 24.21 | |||
