Figure 2 | Scientific Reports

Figure 2

From: Development of a Loop-mediated isothermal amplification (LAMP) technique for specific and early detection of Mycobacterium leprae in clinical samples

Figure 2

Gel electrophoresis analysis of amplification reactions under different LAMP parameters. (a) Temperature optimization. Lanes: (M) Ladder, (1) 65 °C, (2) 66 °C, (3) 67 °C, (4) 68 °C, (5) 69 °C and (6) 70 °C (b) Reaction time optimization. Lanes: (1) 0 min, (2) 15 min, (3) 30 min, (4) 45 min and (5) 60 min, (c) Template concentration optimization. Lanes: (1) 1 ng, (2) 0.5 ng, (3) 0.1 ng, (4) 0.05 ng, (5) 0.01 ng, (6) 5 pg, (7) 1 pg, (d) Comparison of reaction heat source. Lanes 1–3: Heat block, Lanes 4–6: Water bath. (Lanes 1 & 4) ML DNA, (Lanes 2 & 5) MTB control, (Lanes 3 & 6) E.coli control, Lane M ladder, (e) Specificity of 16S rRNA-S primers. Lanes 1–3: 16S rRNA-S primers, Lanes 4–6: 16S rRNA-N primers. (Lanes 1 & 4) ML DNA, (Lanes 2 & 5) MTB control, (Lanes 3 & 6) E.coli control, Lane M is ladder.

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