Figure 1

Total Ion Chromatogram (TIC) of 95% ethanolic ADPE obtained using LC–MS. A 5 mL sample was loaded on Silica C18 Reversed Phase column (150 × 2.1, 2.6 μm), with 0.45 mL min−1 flow rate using mobile phase solvents (a): acetonitrile/water (5:95, v/v), (b): acetonitrile, (c): methanol and (d): 5 mM ammonium acetate (95:5 H2O:Acetonitrile, pH 6.5). Mass spectrometry was performed by ESI and MS data was recorded in the mass range m/z 200–2000 from 0–30 min under ionization mode of ES+ and ES−. Y-axis is relative abundance and X-axis is retention time.