Figure 6 | Scientific Reports

Figure 6

From: Phytochemicals from Ajwa dates pulp extract induce apoptosis in human triple-negative breast cancer by inhibiting AKT/mTOR pathway and modulating Bcl-2 family proteins

Figure 6

Western blot analysis of apoptotic proteins and signaling molecules in MDA-MB-231 cells. (a) Immunoblot analysis showing expression levels of p53, Bax, Bcl2, cleaved Caspase-3, mTOR, p-mTOR, AKT and p-AKT. The MDA-MB-231 cells were treated at two effective concentrations of ADPE (15 and 20 mg/mL) for 48 h. Equal amounts of total proteins (30 μg/lane) were subjected to 10–12% SDS-PAGE. Expression of p53, Bax, Bcl2, cleaved Caspase-3, mTOR, p-mTOR, AKT, p-AKT and β-actin were detected using specific antibodies. Lane 1: 0 mg/mL (Untreated); Lane 2: 15 mg/mL; Lane 3: 20 mg/mL of ADPE. (b) Graph representing relative intensity of various proteins vs concentration. The data represents mean ± SEM of three independent experiments.

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