Figure 5
From: A human infertility-associated KASH5 variant promotes mitochondrial localization
L535Q KASH5 reduces mitochondrial function, but does not impair membrane potential. (A) Colocalization between Mitotracker Red CMXROS and L535Q or L535N KASH5. Scale bar 10 μm. (B) Mitochondrial function assessed by measuring the oxygen consumption rate (OCR) in mitochondria from U2OS cells transfected with an empty vector, wild-type or L535Q KASH5. Black arrows indicate the time of addition of each mitochondrial functional modifier: Oligomycin, FCCP, Rotenone & Antimycin A. Data are expressed as mean ± standard error. (C) Basal respiration, Spare respiration capacity, proton leak and adenosine triphosphate (ATP) production and spare respiratory capacity of the cells. Error bars represent standard error. P < 0.05. The results are the average of two biological replicates and 30 technical replicates for each sample.
