Figure 6
From: A human infertility-associated KASH5 variant promotes mitochondrial localization
Addition of alanine to the KASH domain does not disrupt L535Q mitochondrial targeting. (A) U2OS cells grown on coverslips were transfected with an N-terminal GFP-tagged version of wild-type +Ala, L535Q or L535Q + Ala KASH5. Mitochondria localization was assessed with an anti-TOM20 antibody (pink). (B) Pearson’s correlation coefficient between GFP-KASH5 and TOM20 N = 58. The experiment was repeated four times.
