Figure 2

A typical example of hyperpolarized [1-¹³C]pyruvate metabolic conversion in cerebrum slices over the time course of one recording, shown as a stack plot of ¹³C NMR spectra. The [1-¹³C]lactate, [1-¹³C]pyruvate-hydrate, and [1-¹³C]pyruvate signals were obtained with one product selective pulse and the [¹³C]bicarbonate and [1-¹³C]pyruvate signals with a consecutive product selective pulse. The two pulses were consecutively applied throughout the measurement using an interleaved pulse sequence. For demonstrative purpose we combined the spectra of both interleaved pulses into one stacked 3D plot and omitted the [1-¹³C]pyruvate signal from the [¹³C]bicarbonate spectra. The [1-¹³C]pyruvate and [1-¹³C]pyruvate-hydrate signals were truncated to allow dynamic range for visibility of the [¹³C]bicarbonate signal. The black bar on the right indicates the duration of constant concentration of hyperpolarized [1-¹³C]pyruvate in the hyperpolarized media perfusing the slices.