Figure 2
(a) Copy numbers of small subunit ribosomal DNA (SSU rDNA) (mean = red dot) of T. suzukii in single D. suzukii (SWD) larvae (orange), pupae (blue) and adults (green). The course of infection for second instar larvae (L2) inoculated with 4 × 104 spores. Replicates/numbers (R/N): larvae 3–5: R/N = 2/4, pupae 5–8: R/N = 2/4, pupae 8–13: R/N = 3/5, adult 13–20: R/N = 3/6. GLM analysis for gamma distribution with log-link: χ2 = 14.014, DF = 3, P = 0.003, Tukey test: 3–5 and 13–20: P < 0.001, 5–8 and 13–20: P = 0.252, 8–13 and 13–20: P = 0.305. Different letters indicate statistical difference. Boxplot indicates the median logarithmic DNA quantity per individual (horizontal bar), mean (red dot), error bars (black hinge), asymptotic upper and lower confidence limits (red hinge) outliers (white dot). (b) Bright-field light microscopy of Giemsa-stained smears. Developmental stages of T. suzukii isolated from SWD larvae, pupae and adults at different time points post inoculation. SWD were inoculated in L2 larval stage with 4 × 104 T. suzukii spores. (1) Larval stage (6 dpi). (2) Early pupal stage (8 dpi). (3) Pupal stages (10 dpi). (4) Late pupal stage (13 dpi). (5) Early adult stage (15 dpi). (6) Adult stage (17 dpi). Abbreviations: (M) meront containing two nuclei, (St) sporont, (Sp) spore, (dSt) dividing sporont, (Sb) sporoblast. Scale bar = 5 µm.
